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Table 1 Isolating and characterizing mesenchymal stem cell-derived extracellular vesicles

From: Methods and efficacy of extracellular vesicles derived from mesenchymal stromal cells in animal models of disease: a preclinical systematic review protocol

Question of interest

Example answers

Terminology used

Extracellular vesicles

Exosomes

Microvesicles

Small EVs

Large EVs

Microparticles

Size of EVs

Small EVs (30–150 nm)

Large EVs (150–1000 nm)

Both used together

Both used separately

Method of EV enrichment

Ultracentrifugation

Isolation kit

Tangential flow filtration

Method of EV characterization

Nanoparticle tracking analysis (size)

Dynamic light scattering (size)

Tunable resistive pulse sensing (size)

Western blot (protein content)

Flow cytometry (protein content)

ELISA (protein content)

Proteomics (protein content)

Electron microscopy (morphology)

Bradford/BCA assay

Protein markers used

CD63 (seen in many EVs)

CD9 (seen in many EVs)

CD81 (small EV)

TSG101 (small EV)

Other (specify)

Negative protein markers

Calnexin

Cytochrome C

  1. EV, extracellular vesicle; ELISA, enzyme-linked immunosorbent assay; BCA assay, bicinchoninic acid assay; TSG101, tumor susceptibility gene 101