Question of interest | Example answers |
---|---|
Terminology used | Extracellular vesicles |
Exosomes | |
Microvesicles | |
Small EVs | |
Large EVs | |
Microparticles | |
Size of EVs | Small EVs (30–150 nm) |
Large EVs (150–1000 nm) | |
Both used together | |
Both used separately | |
Method of EV enrichment | Ultracentrifugation |
Isolation kit | |
Tangential flow filtration | |
Method of EV characterization | Nanoparticle tracking analysis (size) |
Dynamic light scattering (size) | |
Tunable resistive pulse sensing (size) | |
Western blot (protein content) | |
Flow cytometry (protein content) | |
ELISA (protein content) | |
Proteomics (protein content) | |
Electron microscopy (morphology) | |
Bradford/BCA assay | |
Protein markers used | CD63 (seen in many EVs) |
CD9 (seen in many EVs) | |
CD81 (small EV) | |
TSG101 (small EV) | |
Other (specify) | |
Negative protein markers | Calnexin |
Cytochrome C |