Skip to main content

Table 3 Studies comparing F. nucleatum load in colorectal specimens of colorectal cancer cases and controls

From: Detection of Fusobacterium nucleatum in feces and colorectal mucosa as a risk factor for colorectal cancer: a systematic review and meta-analysis

First author (year)

Specimen type (No. of specimens CRC/C)

F. nucleatum quantification measures reported

Statistics reported

F. nucleatum load in CRC compared to C

C

CRC

p

Relative abundance

 Zhang (2018) [42]

Feces (130/130)

Relative contribution to total bacteria (%)

Mean

0.001

0.17

< 0.001

 Vogtmann (2016) [53]

Feces (52/53)

Relative contribution to total bacteria (%)

Mean

0.003

0.08

0.043

 Wang (2012) [55]

Feces (46/56)

Relative contribution to total bacteria (%)

Mean

0.002

0.061

0.005

Absolute quantification

 Yu (2017) [60]

Feces (74/54)

Bacteria counts

Rank mean

45.09 IMG

40.32 MLG

54.62 OTU

78.66 IMG

82.14 MLG

71.71 OTU

< 0.001

 Suehiro (2017) [45]

Feces (158/60)

Absolute copy number of F. nucleatum

Median (min–max)

17.5 (0–5793)

317 (0–17,343)

< 0.0001

 Fukugaiti (2015) [44]

Feces (7/10)

Log no. copies of F. nucleatum/gram

Mean ± SD (min-max)

4.0 ± 1.5 (1.0–6.4)

6.2 ± 1.5 (3.5–8.0)

0.01

 Mira-Pascual (2015) [50]

Feces (6/10)

Log no. gene copies of F. nucleatum/mg

Median (IQR)

4.16 (3.47–4.85)

4.70 (3.85–5.15)

---

 Rezasoltani (2018) [46]

Feces (20/31)

Cq value (Quantity of F. nucleatum = 10 (\( \frac{Cq-b}{m} \) ))

Mean (SD)

29.16 (3.31)

17.74 (3.59)

< 0.05

 Liu (2020) [62]

Feces (53/45)

Log 10 copies of F. nucleatum ∕ gram

Mean (SD)

≈3.5 (1)

≈6 (1)

< 0.01

Relative quantification

 Russo (2017) [52]

Feces (10/10)

Ratio: \( \frac{Cq\kern0.5em F. nucleatum\kern0.75em }{Cq\ total\ bacteria} \)

Mean

≈2.25

≈1.8

> 0.05

 Yu (2015) [41]

Feces (42/52)

ΔCq value

Median [IQR]

≈26 [2]

≈19 [2]

< 0.001

Biopsy (31/37)

≈27 [2]

≈18 [3]

< 0.001

 Flanagan (2014) [43]

Feces (7 /25)

2−ΔCq value

Median

2−21

2−15

0.02

 Liang (2017) [49] Coh I

Feces (170/200)

2−ΔCq value

Median

≈2−17

≈2−5

< 0.0001

 Liang (2017) [49] Coh II

Feces (33/36)

2−ΔCq value

Median

≈2−16

≈2−13

0.012

 Eklof (2017) [47]

Feces (39/66)

Log 2−ΔCq

Median [IQR]

≈− 10 [2]

≈− 7 [5]

< 0.001

 Guo (2018) [48]

Feces (215/156)

Log 2−ΔCq

Mean

≈−4

≈− 3

< 0.0001

 Wong (2017) [56]

Feces (104/102)

Log 2−ΔCq (fold change from HC to CRC)

Mean

≈−7

≈−5 (132)

< 0.001

 Xie (2017) [58]

Feces (327/242)

Log2 RQ, based on ΔCq (Fold change from HC to CRC)

Mean

≈−20

≈− 16 for early stage and −18 for advanced stage (5.12)

0.006

< 0.001

 Tunsjo (2019) [61]

Feces (23/22)

2−ΔCq value (fold change from HC to CRC)

Median

≈2−7

≈2−5

(66)

0.0073

  1. CRC colorectal cancer cases, C controls (colorectal-adenoma- and CRC-free CRC and C subjects), SD standard deviation, Coh cohort, OTUs operational taxonomic units, MLGs metagenomic linkage groups, IMG integrated microbial genome, Cq quantification cycle in qPCR, ΔCq the average Cq value of F. nucleatum—the average Cq value of total bacteria (or reference gene), min minimum, max maximum
  2. ---Not reported
  3. ≈A value read from a graph in the study material