Table 2 Summary of study characteristics of included studies
From: A systematic review of enteric dysbiosis in chronic fatigue syndrome/myalgic encephalomyelitis
Author | Year | Study design | Sample type | Dx | Country | Samples size | Method of analysing microbiome | Quality score | |
|---|---|---|---|---|---|---|---|---|---|
CFS/ME | Control | ||||||||
Armstrong et al. | 2016 | Observational case-control | CFS/ME | Canadian Criteria (2003) | Australia | 34 F | 25 F | Bacterial culture and MALDI-TOF MS | 73% (good) |
Frémont et al. | 2013 | Observational case-control | CFS/ME | Fukuda (1994) | Belgium | Belgian: 15 F, 3 M; Norwegian: 22 F, 3 M | Belgian: 15 F, 4 M; Norwegian: 14F, 3 M | PCR amplification and high-throughput sequencing of 16S rRNA genes | 67% (fair) |
Giloteauc et al. | 2016 | Observational case-control | CFS/ME | Fukuda (1994) | USA | 38 F, 11 M | 30 F, 9 M | 16S rRNA genes sequenced from faecal samples | 47% (poor) |
Mandarano et al. | 2018 | Observational case-control | CFS/ME | Fukuda (1994) | USA | Taxa abundance comparisons: 13 F, 4 M; diversity subgroup: 7 F, 4 M | Taxa abundance comparisons: 16 F, 1 M; diversity subgroup: 9 F, 1 M | DNA extraction, 18S amplification, sequencing using QIIME | 47% (poor) |
Rao et al. | 2009 | RCT, pilot study | CFS/ME | Canadian Criteria (2003) | Canada | CFS/ME 27 F, 8 M: 16 placebo, 19 treatment (Lactobacillus casei strain Shirota) | Culture technique | 56% (fair) | |
Sheedy et al. | 2009 | Observational case-control | CFS/ME | Holmes (1988) Fukuda (1994) Canadian Criteria (2003) | Australia | 108 | 177 | Culture technique | 80% (good) |
Shukla et al. | 2015 | Observational case-control | CFS/ME | Fukuda (1994) | Italy | 8 F, 2 M | 8 F, 2 M | 16S rRNA amplification and pyrosequencing | 80% (good) |